Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography AngiographyQiang Zeng 1, Doug A. Gouchoe 2,3, Mahboubeh Nabavinia 4, Yong Gyu Lee 2, Xi Wang 2, Terri A. Shaffer 5, Mitchel R. Stacy 4,5,6, Blake R. Peterson 7,8, Bryan A. Whitson 2,9, Christopher Breuer 1,4, Sylvester M. Black 1,10
1Department of Surgery, Nationwide Children’s Hospital, 2COPPER Laboratory, The Ohio State University, 388th Surgical Operation Operations Squadron, WPAFB, 4Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, 5Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, 6Interdisciplinary Biophysics Graduate Program, The Ohio State University, 7Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, 8The Ohio State University Comprehensive Cancer Center, 9Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, 10Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center
In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.
