Anmelden

ICAR-Research Complex for Eastern Region

1 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Praapti Jayaswal 1, Mohd Ilyas 1, Kuljit Singh 1,2, Saurabh Kumar 1,3, Lovely Sisodiya 1, Sapna Jain 1, Rahul Mahlawat 1, Nishant Sharma 1,4, Vishal Gupta 1, Krishnamohan Atmakuri 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.

JoVE Logo

Datenschutz

Nutzungsbedingungen

Richtlinien

Forschung

Lehre

ÜBER JoVE

Copyright © 2024 MyJoVE Corporation. Alle Rechte vorbehalten