Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA SequencingLine O. Elingaard-Larsen 1,2, Katie L. Whytock 1, Adeline Divoux 1, Meghan Hopf 1, Erin E. Kershaw 3, Jamie N. Justice 4, Bret H. Goodpaster 1, Nancy E. Lane 5, Lauren M. Sparks 1
1Translational Research Institute, AdventHealth, 2Steno Diabetes Center Copenhagen, 3Division of Endocrinology and Metabolism, University of Pittsburgh, 4Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, 5Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health
The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.
