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United States Air Force Academy Life Sciences Research Center

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Production and Analysis of Sporosarcina pasteurii Biocement Bricks Using Custom 3D-Printed Molds for Unconfined Compression Tests
Victoria L. Morrison 1, Morgan T. Vance 1, Melanie L. M. Grogger 2, Hannah G. Grover 1, J. Jordan Steel 1
1Biology Department, United States Air Force Academy, 2United States Air Force Academy Life Sciences Research Center

Sporosarcina pasteurii is a ureolytic bacterium that breaks down urea into carbonate and ammonium. The carbonate combines with calcium to form calcium carbonate, creating a crystal lattice that anchors surrounding particles together to produce biocement. This is a convenient protocol for using 3D-printed molds to create biocement bricks suitable for compression testing.

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