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Dartmouth Cancer Center

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Developmental Biology

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
Jacob Buford 1, Owen Wilkins 2, Fred Kolling IV 2, Steven D. Leach 2, Duncan Morhardt 1,3
1Department of Surgery, Dartmouth Hitchcock Medical Center, 2Dartmouth Cancer Center, 3Dartmouth College, Geisel School of Medicine

Here, we present a method for aligning and cryosectioning multiple Zebrafish (Danio rerio) larvae samples and collecting them on a single slide for spatial transcriptomic analysis.

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