Austin Health

2 ARTICLES PUBLISHED IN JoVE

Biology

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Adam C. Parslow^1,2, Andrew H.A. Clayton³, Peter Lock⁴, Andrew M. Scott^1,2,5,6,7

¹Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, ²School of Cancer Medicine, La Trobe University, ³Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, ⁴LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, ⁵Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, ⁶Department of Medicine, University of Melbourne, ⁷Department of Molecular Imaging and Therapy, Austin Health

Antibodies that bind to target receptors on the cell surface can confer conformation and clustering alterations. These dynamic changes have implications for characterizing drug development in target cells. This protocol utilizes confocal microscopy and image correlation spectroscopy through ImageJ/FIJI to quantify the extent of receptor clustering on the cell surface.

Cancer Research

Using 16α-[18F]-Fluoro-17β-Estradiol PET to Visualize Estrogen Receptor α Expression in Human Breast Cancer Xenografts in Female Ovariectomized Mice

Sadia Quazi^1,2, Nhi Huynh¹, Angela Rigopoulos¹, Alexander McDonald^1,3, Uwe Ackermann³, Andrew Mark Scott^1,2,3,4, Ingrid Julienne Georgette Burvenich^1,2

¹Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, ²School of Cancer Medicine, La Trobe University, ³Department of Molecular Imaging and Therapy, Austin Health, ⁴Faculty of Medicine, The University of Melbourne

Here, we demonstrate a protocol to use 16α-[18F]-fluoro-17β-estradiol (¹⁸F-FES) positron emission tomography (PET) as a tool to visualize ERα expression in ERα-positive breast xenografts.