Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow CytometryYashdeep Phanse 1, Amanda E. Ramer-Tait 1, Sherree L. Friend 2, Brenda Carrillo-Conde 3, Paul Lueth 1, Carrie J. Oster 1, Gregory J. Phillips 1, Balaji Narasimhan 3, Michael J. Wannemuehler 1, Bryan H. Bellaire 1
1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University
In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.
