Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCRSonia Apaza 1, Susan Espetia 1, Robert H. Gilman 1,2, Sonia Montenegro 3, Susana Pineda 3, Fanny Herhold 1, Romeo Pomari 1, Margaret Kosek 2, Nancy Vu 1, Mayuko Saito 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine
A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.
