Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric SpectroscopyMarie-Yvonne Akoume 1, Anita Franco 1, Alain Moreau 1,2
1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases, Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine, Université de Montréal
A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.
