Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect TissuesAdi Kliot 1,2, Svetlana Kontsedalov 1, Galina Lebedev 1, Marina Brumin 1, Pakkianathan Britto Cathrin 1, Julio Massaharu Marubayashi 1, Marisa Skaljac 1,3, Eduard Belausov 4, Henryk Czosnek 2, Murad Ghanim 1
1Department of Entomology, Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 3Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences, Volcani Center
We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.
