national and kapodistrian university of athens

2 ARTICLES PUBLISHED IN JoVE

Biology

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Anna Hadjihambi^*1,2, Rallia-Iliana Velliou^*3, Panagiotis Tsialios⁴, Aigli-Ioanna Legaki³, Antonios Chatzigeorgiou^*3, Maritina G. Rouchota^*4

¹The Roger Williams Institute of Hepatology London, Foundation for Liver Research, ²Faculty of Life Sciences and Medicine, King's College London, ³Department of Physiology, Medical School, National and Kapodistrian University of Athens, ⁴BIOEMTECH, Lefkippos Attica Technology Park NCSR "Demokritos"

Using a diet-induced non-alcoholic fatty liver disease (NAFLD) mouse model, we describe the use of novel in vivo micro-computed tomography imaging techniques as a non-invasive method to assess the progression stages of NAFLD, focusing predominantly on the hepatic vascular network due to its significant involvement in NAFLD-related hepatic dysregulation.

Neuroscience

The Mouse Stroke Unit Protocol with Standardized Neurological Scoring for Translational Mouse Stroke Studies

Michail Georgopoulos¹, Angelos Pavlopoulos¹, Nefeli Zerva¹, Asterios Kokkonakis¹, Iordanis Mourouzis¹, Nikolaus Plesnila², Constantinos Pantos¹, Athanasios Lourbopoulos^1,2,3

¹Department of Pharmacology Medical School of Athens, National and Kapodistrian University of Athens, ²Institute for Stroke and Dementia Research (ISD), University of Munich Medical Center, ³Department of Neurointensive Care, Schoen Clinic Bad Aibling

This article presents a protocol for executing the highly translational and independently proven mouse Stroke Unit (mSU) concept in large-stroke mice. It also provides a standardized protocol for executing the focal experimental stroke scale in mice, which evaluates stroke focal deficits detected even long-term.