RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound HealingAeri Yoo *1, Hyun-Min Park *2, Soon-Suk Kang 2, Eun-Soon Kim 2, Hungwon Tchah 2, Jae Yong Kim 2
1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center
Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.
