Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical MethodLilia Tierrablanca-Sánchez 1, Víctor Pérez Medina Martínez 1, Nancy D. Ramírez Ibañez 1, Néstor O. Pérez Ramírez 1, Francisco Luis Flores Ortiz 1, Emilio Medina-Rivero 2
1Research and Development, Probiomed, 2Bioprocess Research and Development Unit, Instituto Politecnico Nacional
A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.
