Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In VitroVincent van Duinen 1,2, Wendy Stam 1, Viola Borgdorff 3, Arie Reijerkerk 3, Valeria Orlova 4, Paul Vulto 5, Thomas Hankemeier 2, Anton Jan van Zonneveld 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas
This method describes the culture of iPSC-derived endothelial cells as 40 perfused 3D microvessels in a standardized microfluidic platform. This platform enables the study of gradient-driven angiogenic sprouting in 3D, including anastomosis and stabilization of the angiogenic sprouts in a scalable and high-throughput manner.
