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4 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

Daxiang Li¹, Jian Wu¹, Yan Bai¹, Xiaochen Zhao², Lijun Liu¹

¹Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, ²Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences

We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

Behavior

Tactile Vibrating Toolkit and Driving Simulation Platform for Driving-Related Research

Ao Zhu¹, Annebella Tsz Ho Choi¹, Ko-Hsuan Ma¹, Shi Cao², Han Yao¹, Jian Wu³, Jibo He¹

¹Psychology Department, School of Social Sciences, Tsinghua University, ²Department of Systems Design Engineering, University of Waterloo, ³Haier Innovation Design Center, Haier Company

This protocol describes a driving simulation platform and a tactile vibrating toolkit for the investigation of driving-related research. An exemplar experiment exploring the effectiveness of tactile warnings is also presented.

Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones

Yincheng Wang¹, Ke Wang¹, Yuqi Huang¹, Di Wu², Jian Wu³, Jibo He¹

¹Department of Psychology, School of Social Sciences, Tsinghua University, ²Department of Computer Science, Beijing Normal University, ³Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

Biology

Surgically Induced Cardiac Volume Overload by Aortic Regurgitation in Mouse

Jian Wu^*1, Shijun Wang^*1, Le Kang^*1, Chunjie Yang^*1, Jianguo Jia¹, Xingxu Wang², Ge Wei¹, Xuan Li¹, Jieyun You², Yunzeng Zou¹

¹Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, ²Department of Cardiovascular Medicine, Shanghai East Hospital, School of Medicine, Tongji University

This protocol presents a practical guide on the surgery for creation of aortic regurgitation (AR) in the mouse. Assessment of the AR mouse by echocardiography and invasive hemodynamic measurement recapitulates its clinically relevant characteristics of volume overload-induced eccentric hypertrophy, suggesting its promising application in the study of cardiac hypertrophy.