A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its MalignanciesMukti R. Parikh 1, Andrew R. Belch 2,3, Linda M Pilarski 2,3, Julia Kirshner 1
1Department of Biological Sciences, Purdue University, 2Department of Oncology, University of Alberta, 3Cross Cancer Institute
In standard culture methods cells are taken out of their physiological environment and grown on the plastic surface of a dish. To study the behavior of primary human bone marrow cells we created a 3-D culture system where cells are grown under conditions recapitulating the native microenvironment of the tissue.
