University of Tabuk

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies

Lee-Hwa Tai¹, Christiano Tanese de Souza¹, Shalini Sahi¹, Jiqing Zhang^1,2,4, Almohanad A Alkayyal^1,3,5, Abhirami Anu Ananth^1,3, Rebecca A.C. Auer^1,6

¹Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine, University of Ottawa, ³Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ⁴Department of Neurosurgery, The Second Hospital of Shandong University, ⁵Department of Medical Laboratory Technology, University of Tabuk, ⁶Department of Surgery, Ottawa General Hospital

A mouse tumor model of surgical stress is used to explore how postoperative immune suppression promotes metastatic disease and to evaluate immunostimulatory perioperative therapies.

Biology

Preparation of Agar Bead Embedded Mycobacterium abscessus to Inoculate Immunocompetent Mice Intratracheally

Julianna Proietto^*1, Betelhem Tatek^*1, Camilla Folvar^*1, Diana C. Jimenez², Ilham M. Alshiraihi^2,3, Mercedes Gonzalez-Juarrero², Fabio Saliu⁴, Federica Vacca⁴, Daniela Maria Cirillo⁴, Nicolà Lore⁴, Thomas Dick^1,5,6, Véronique Dartois^1,5, Wassihun Wedajo Aragaw¹

¹Center for Discovery and Innovation, Hackensack Meridian Health, ²Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, ³Department of Biological Sciences, College of Science, University of Tabuk, ⁴Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, ⁵Department of Medical Sciences, Hackensack Meridian School of Medicine, ⁶Department of Microbiology and Immunology, Georgetown University

Mice are generally resistant to infections by Mycobacterium abscessus, which complicates the discovery and development of much-needed antibiotics against pulmonary infections. Here, we describe a method of inoculum preparation and intratracheal infection that was shown to deliver sustained infection in immunocompetent mice.