Deriving De Novo Atomic Models by Cryo Electron MicroscopyPeng Ge 1, Nicole Poweleit 1,2, Robert P Gunsalus 2,3, Hong Zhou 1,2
1Electron Imaging Center for Nanomachines (EICN), California NanoSystems Institute, University of California, 2Immunology and Molecular Genetics, University of California, 3UCLA Institute of Genomics and Proteomics
Cryo electron microscopy (cryoEM) can be employed to derive de novo atomic models of macromolecular complexes in solution. The steps involved in high resolution cryoEM of biological molecules, from image recording, to data processing, to atomic modeling based on the resulting cryoEM density map, are illustrated.
