Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid TumorsHanibal Bohnenberger 1, Philipp Ströbel 1, Sebastian Mohr 2, Jasmin Corso 3, Tobias Berg 2, Henning Urlaub 3,4, Christof Lenz 3,4, Hubert Serve 2,5,6, Thomas Oellerich 2,5,6
1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center
In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.
