Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral VectorAnke Van der Perren 1, Cindy Casteels 2, Koen Van Laere 2, Rik Gijsbers 3,4, Chris Van den Haute 1,4, Veerle Baekelandt 1
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, 2Division of Nuclear Medicine, Leuven University Hospital - KU Leuven, 3Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 4Leuven Viral Vector Core, KU Leuven
This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.
