Preparation of Extracellular Matrix Protein Fibers for Brillouin SpectroscopyRyan S. Edginton 1, Sara Mattana 2, Silvia Caponi 2,3, Daniele Fioretto 2, Ellen Green 1, C. Peter Winlove 1, Francesca Palombo 1
1School of Physics and Astronomy, University of Exeter, 2Department of Physics and Geology, University of Perugia, 3Istituto Officina dei Materiali del CNR, Unità di Perugia
We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.
