Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human CellsHuan-Huan Wei *1, Yuanlong Liu *1, Yang Wang 2, Qianyun Lu 1, Xuerong Yang 1, Jiefu Li 1, Zefeng Wang 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University
This report describes a bioengineering method to design and construct novel Artificial Splicing Factors (ASFs) that specifically modulate the splicing of target genes in mammalian cells. This method can be further expanded to engineer various artificial factors to manipulate other aspects of RNA metabolism.
