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Ghent University (UGent)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Judith Verhelst^1,2, Dorien De Vlieger^1,2, Xavier Saelens^1,2

¹Inflammation Research Center, VIB, ²Department of Biomedical Molecular Biology, Ghent University

This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.

Immunology and Infection

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

Lien Van Hoecke^1,2, Emma Richelle Job^1,2, Xavier Saelens^1,2, Kenny Roose^1,2

¹UGent Center for Medical Biotechnology, VIB, ²Department of Biomedical Molecular Biology, Ghent University (UGent)

The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.

Neuroscience

Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus

Elien Van Wonterghem^*1,2, Lien Van Hoecke^*1,2, Griet Van Imschoot^1,2, Daan Verhaege^1,2, Marlies Burgelman^1,2, Roosmarijn E. Vandenbroucke^1,2

¹VIB Center for Inflammation Research, VIB, ²Department of Biomedical Molecular Biology, Ghent University

The choroid plexus (CP), an understudied tissue in neuroscience, plays a key role in health and disease of the central nervous system. This protocol describes a microdissection technique for isolating the CP and the use of scanning electron microscopy to obtain an overall view of its cellular structure.