Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA DamageCheryl A. Hobbs *1, Leslie Recio 1, John Winters 1, Kristine L. Witt 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.
