X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050Raphael Dutoit 1,2, Nathalie Brandt 2, Dany Van Elder 1, Louis Droogmans 1
1Laboratory of Microbiology, Department of Molecular Biology, Université Libre de Bruxelles, 2Labiris Institut de Recherche
This protocol has been developed to study the dimer-dodecamer transition of TmPep1050, an M42 aminopeptidase, at the structural level. It is a straightforward pipeline starting from protein purification to X-ray data processing. Crystallogenesis, data set indexation, and molecular replacement have been emphasized through a case of study, TmPep1050H60A H307A variant.
