Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse BrainRebecca Z. Weber 1,2, Chantal Bodenmann 1, Daniela Uhr 1, Kathrin J. Zürcher 1, Debora Wanner 1, Melanie Generali 1, Roger M. Nitsch 1, Ruslan Rust *1,2, Christian Tackenberg *1,2
1Institute for Regenerative Medicine, University of Zurich, 2Neuroscience Center Zurich, University of Zurich and ETH Zurich
We describe the intraparenchymal transplantation of human neural progenitor cells transduced with a dual reporter vector expressing luciferase-green fluorescent protein (GFP) in the mouse brain. After transplantation, the luciferase signal is repeatedly measured using in vivo bioluminescence and GFP-expressing grafted cells identified in brain sections using fluorescence microscopy.
