Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human TherapyMartino Guiotto *1,4, Silvia Palombella *2, Stefania Brambilla 2, Lee Ann Applegate 3, Mathis Riehle 4, Andrew Hart 4, Wassim Raffoul 1, Pietro Giovanni di Summa 1
1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab, IRCCS Ospedale Galeazzi Sant'Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, Lausanne University Hospital, 4Centre for the Cellular Microenvironment, University of Glasgow
Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.
