biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

large animal models

This collection features research methods using porcine, bovine, equine, and leporine (rabbit) models at their embryonic, neonatal, and adult stages. These techniques include ex-vivo and in-vivo studies, organ harvesting and preservation methods, cell isolation and culturing methodologies, microbial culturing, biochemical and biophysical assays, diagnostic tests, and various surgical procedures.

Large Animal Models

drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Drosophila melanogaster (fruit fly)

caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Caenorhabditis elegans (worm)

danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Danio rerio (zebrafish)

rodent models

This collection features research methods using rodent models in embryonic, neonatal, and adult stages. The techniques include in vitro and in vivo organ perfusion, tissue and organ isolation, microbial culturing, biochemical studies, organ transplant methodologies, behavioral tests, and surgical procedures.

Rodent Models

embryo

Embryo

larva

Larva

adult

Adult

embryo-based chemical toxicity screen: assessing effects on developing zebrafish embryos

embryo-based-chemical-toxicity-screen-assessing-effects-on-developing

Embryo-Based Chemical Toxicity Screen: Assessing Effects on Developing Zebrafish Embryos

Source: Hao J. et al., Large Scale Zebrafish-Based In vivo Small Molecule Screen. J. Vis. Exp. (2010)
In this article, zebrafish embryos are used for ...

mating and egg staging: a method to generate embryos and sort them by developmental stage

mating-egg-staging-method-to-generate-embryos-sort-them-developmental

Mating and Egg Staging: A Method to Generate Embryos and Sort Them by Developmental Stage

Source: Hostelley T.L., et al. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (2017)
The article ...

light sheet microscopy sample preparation: mounting live zebrafish embryos for long-term imaging

light-sheet-microscopy-sample-preparation-mounting-live-zebrafish

Light Sheet Microscopy Sample Preparation: Mounting Live Zebrafish Embryos for Long-Term Imaging

Source: Icha J., et al. Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development. J. Vis. Exp. (2016).
This article describes sample ...

layered agar mounting: preparing live zebrafish embryos for long-term imaging with an inverted microscope

layered-agar-mounting-preparing-live-zebrafish-embryos-for-long-term

Layered Agar Mounting: Preparing Live Zebrafish Embryos for Long-Term Imaging with an Inverted Microscope

Source: Upadhyay S., et.al. A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos. J. Vis. Exp. (2020).
This ...

agar mounting: a basic method of mounting live zebrafish embryos for long-term imaging

agar-mounting-basic-method-mounting-live-zebrafish-embryos-for-long

Agar Mounting: A Basic Method of Mounting Live Zebrafish Embryos for Long-Term Imaging

Source: Hagedorn E. J., et al. Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae. J. Vis. Exp. (2016).
This video ...

two-photon laser axotomy: a method to injure axons in zebrafish embryos and observe axonal recovery

two-photon-laser-axotomy-method-to-injure-axons-zebrafish-embryos

Two-Photon Laser Axotomy: A Method to Injure Axons in Zebrafish Embryos and Observe Axonal Recovery

Source: O&#39;Brien G. S., et al. Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos. J. Vis. Exp. (2009).
This video describes ...

whole-cell patch clamp electrophysiology: a method to study electrical properties of neurons

whole-cell-patch-clamp-electrophysiology-method-to-study-electrical

Whole-Cell Patch Clamp Electrophysiology: A Method to Study Electrical Properties of Neurons

Source: Roy, B., et al. Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells. J. Vis. Exp. (2013).
This protocol describes a technique called ...

embryo microinjection: a technique to deliver a compound into the zebrafish yolk

embryo-microinjection-technique-to-deliver-compound-into-zebrafish

Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk

Source:&#160; Sorlien, E. L., et al. Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio. J. ...

photomotor response assay: a method to measure the behavioral response of larval zebrafish to a sudden change in lighting condition

photomotor-response-assay-method-to-measure-behavioral-response

Photomotor Response Assay: A Method to Measure the Behavioral Response of Larval Zebrafish to a Sudden Change in Lighting Condition

Source: Steele, W. B., et al. Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant ...

prey capture assay: a method to study the prey capture behavior of zebrafish larva

prey-capture-assay-method-to-study-prey-capture-behavior-zebrafish

Prey Capture Assay: A Method to Study the Prey Capture Behavior of Zebrafish Larva

Source: Muto, A., et al. Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in ...

escape response assay: a method to study the response of zebrafish larva to touch stimuli

escape-response-assay-method-to-study-response-zebrafish-larva-to

Escape Response Assay: A Method to Study the Response of Zebrafish Larva to Touch Stimuli

Source: Sztal, T. E., et al. Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish.&#160;J. Vis. ...

zebrafish avoidance and thigmotaxis assay: a high-throughput method to examine larval behavior in response to aversive visual stimulus

zebrafish-avoidance-thigmotaxis-assay-high-throughput-method-to

Zebrafish Avoidance and Thigmotaxis Assay: A High-Throughput Method to Examine Larval Behavior in Response to Aversive Visual Stimulus

Source: Richendrfer, H., et al. Automated High-throughput Behavioral Analyses in Zebrafish Larvae. &#160;J. Vis. Exp.&#160;(2013)
The video describes the ...

larval locomotion assay: high-throughput method to study neurobehavioral toxicity of a compound in zebrafish larvae

larval-locomotion-assay-high-throughput-method-to-study

Larval Locomotion Assay: High-Throughput Method to Study Neurobehavioral Toxicity of a Compound in Zebrafish Larvae

Source: Zhang B., et al. Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae. J. Vis. Exp. (2020)
This article describes ...

targeted liver ablation: inducing hepatocyte death with metronidazole in transgenic zebrafish larvae

targeted-liver-ablation-inducing-hepatocyte-death-with-metronidazole

Targeted Liver Ablation: Inducing Hepatocyte Death with Metronidazole in Transgenic Zebrafish Larvae

Source: Choi T.Y., et al. Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration. J. Vis. Exp. (2015)
The article describes ...

zebrafish optogenetics: activating genetically modified somatosensory neuron to study larval behavioral responses

zebrafish-optogenetics-activating-genetically-modified-somatosensory

Zebrafish Optogenetics: Activating Genetically Modified Somatosensory Neuron to Study Larval Behavioral Responses

Source: Palanca A. M. S., et al. Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato. J. Vis. Exp. (2013).
This video describes ...

eye lens dissection: a technique to observe zebrafish lens morphology

eye-lens-dissection-a-technique-to-observe-zebrafish-lens-morphology

Eye Lens Dissection: A Technique to Observe Zebrafish Lens Morphology

Source: Vorontsova I. et al, Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity. J. Vis. Exp. (2019).
This video describes the ...

zebrafish patient-derived xenograft (zpdx): a cancer model

zebrafish-patient-derived-xenograft-zpdx-a-cancer-model

Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model

Source: Wang L. et. al., Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug ...

zebrafish electrocardiography (ecg): a minimally invasive assay to evaluate cardiac function

zebrafish-electrocardiography-ecg-minimally-invasive-assay-to

Zebrafish Electrocardiography (ECG): A Minimally Invasive Assay to Evaluate Cardiac Function

Source: Zhao, Y., et al. In Vivo Surface Electrocardiography for Adult Zebrafish. J. Vis. Exp. (2019).
This video shows how&#160;to perform ...

zebrafish rheotaxis assay: a method for measuring orientation behavior in response to different water flow rates

zebrafish-rheotaxis-assay-method-for-measuring-orientation-behavior

Zebrafish Rheotaxis Assay: A Method for Measuring Orientation Behavior in Response to Different Water Flow Rates

Source: Cresci, A., et al. Assessing the Influence of Personality on Sensitivity to Magnetic Fields in Zebrafish.&#160;J. Vis. Exp.&#160;(2019)
This video ...

light-dark preference test: a method to study anxiety-related behavior in zebrafish

light-dark-preference-test-method-to-study-anxiety-related-behavior

Light-Dark Preference Test: A Method to Study Anxiety-Related Behavior in Zebrafish

Source: Holcombe, A., et al. A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal. &#160;J. Vis. ...

optokinetic response assay: a method to measure visual acuity in zebrafish

optokinetic-response-assay-method-to-measure-visual-acuity

Optokinetic Response Assay: A Method to Measure Visual Acuity in Zebrafish

Source: Cameron, D. J., et al. The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish.&#160;J. Vis. Exp.&#160;(2013)
This video ...

aggression assay: a method to study aggressive behavior of zebrafish in response to a mirror

aggression-assay-method-to-study-aggressive-behavior-zebrafish

Aggression Assay: A Method to Study Aggressive Behavior of Zebrafish in Response to a Mirror

Source: Way G.P., et al. Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes. J. Vis. Exp. (2016)
The article describes an assay ...

novel tank diving assay: a method to study innate stress responses in zebrafish

novel-tank-diving-assay-method-to-study-innate-stress-responses

Novel Tank Diving Assay: A Method to Study Innate Stress Responses in Zebrafish

Source: Chin J.S.R., et al. Behavioral Approaches to Studying Innate Stress in Zebrafish. J. Vis. Exp. (2019)
The article describes a behavioral method to ...

morpholino injection: a method to knockdown target gene expression to study function in zebrafish

morpholino-injection-method-to-knockdown-target-gene-expression-to

Morpholino Injection: A Method to Knockdown Target Gene Expression to Study Function in Zebrafish

Source: Hyde D.R., et al. In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin. J. Vis. Exp. (2012)
The protocol ...

dna extraction from tail clip: a method used in zebrafish genotyping

dna-extraction-from-tail-clip-a-method-used-in-zebrafish-genotyping

DNA Extraction from Tail Clip: A Method Used in Zebrafish Genotyping

Source: Xing L., et al. Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis. J. Vis. Exp. (2014).
This article describes ...

adult zebrafish electrophysiology: a method to measure the extracellular electrical activity of neurons in vivo

adult-zebrafish-electrophysiology-method-to-measure-extracellular

Adult Zebrafish Electrophysiology: A Method to Measure the Extracellular Electrical Activity of Neurons In Vivo

Source: Johnston, L., et al. Electrophysiological Recording in the Brain of Intact Adult Zebrafish. &#160;J. Vis. Exp. (2013)
The protocol describes the ...

in vivo electroporation: a site-specific method to transfect zebrafish

in-vivo-electroporation-a-site-specific-method-to-transfect-zebrafish

In Vivo Electroporation: A Site-Specific Method to Transfect Zebrafish

Source: Durovic, T., et al. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (2019).
This ...

intramuscular (im) injection: compound delivery into the dorsal muscle of an adult zebrafish

intramuscular-im-injection-compound-delivery-into-dorsal-muscle-an

Intramuscular (IM) Injection: Compound Delivery into the Dorsal Muscle of an Adult Zebrafish

Source: Myllym&#228;ki, H., et al. Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines. J. Vis. Exp. (2018).
This video ...

intraperitoneal injection: a method of solution delivery into the abdominal cavity of an adult zebrafish

intraperitoneal-injection-method-solution-delivery-into-abdominal

Intraperitoneal Injection: A Method of Solution Delivery into the Abdominal Cavity of an Adult Zebrafish

Source: Luukinen, H., et al. Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish.  J. Vis. Exp. (2018).
This video describes a ...

intrathoracic injection: compound delivery to the adult zebrafish heart

intrathoracic-injection-compound-delivery-to-the-adult-zebrafish-heart

Intrathoracic Injection: Compound Delivery to the Adult Zebrafish Heart

Source: Bise, T., et al. Intrathoracic Injection for the Study of Adult Zebrafish Heart.  J. Vis. Exp. (2019).
This video describes the technique of ...

adult zebrafish intestine dissection: a technique to analyze compound absorption

adult-zebrafish-intestine-dissection-technique-to-analyze-compound

Adult Zebrafish Intestine Dissection: A Technique to Analyze Compound Absorption

Source: Jie J. et. al., Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds. J. Vis. Exp. (2018).
This ...

heart dissection: a method to observe zebrafish cardiac development

heart-dissection-a-method-to-observe-zebrafish-cardiac-development

Heart Dissection: A Method to Observe Zebrafish Cardiac Development

Source: Singleman C. et. al., Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio. J. Vis. Exp. (2011).
This video describes a technique ...

zebrafish brain dissection: a technique of fish neurobiology

zebrafish-brain-dissection-a-technique-of-fish-neurobiology

Zebrafish Brain Dissection: A Technique of Fish Neurobiology

Source: Lopez-Ramirez M. A. et. al., Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (2016).
This video describes the ...

zebrafish cardiomyopathy model: drug-induced cardiotoxicity

zebrafish-cardiomyopathy-model-drug-induced-cardiotoxicity

Zebrafish Cardiomyopathy Model: Drug-Induced Cardiotoxicity

Source: Ma X., et. al. A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish. J. Vis. Exp. (2018).
This video describes the technique of creating ...

streptozocin treatment: a zebrafish model of type 1 diabetes mellitus

streptozocin-treatment-a-zebrafish-model-of-type-1-diabetes-mellitus

Streptozocin Treatment: A Zebrafish Model of Type 1 Diabetes Mellitus

Source: Intine R. V., et. al. A Zebrafish Model of Diabetes Mellitus and Metabolic Memory. J. Vis. Exp. (2013).
This video describes the technique of ...

Encyclopedia Of Experiments - biology