Preparation of Cell Lysate for Non-denaturing PAGE
4:04
Electrophoresis
4:53
Western Blot
6:34
Results: Human MxA Protein from IFN-α Stimulated A549 Cells is a Tetramer
7:22
Conclusion
Transkript
The overall goal of this experiment is to determine the number of subunits in homooligomeric protein complexes in cell lysates using non-denaturing sodium dodecosulfate-polyacrylamide gel electrophoresis. This method can help answer key questions
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This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.