Results: Observation of Axonal Growth in Micro-TENNs by Phase-contrast Imaging and Cytoarchitecture by Confocal Microscopy
9:51
Conclusion
Transkript
This protocol describes the fabrication of micro-tissue engineered neural networks or micro-TENNs, which are miniature three-dimensional constructs comprised of long aligned axonal tracts spanning discrete neuronal populations within the lumen of
Sign in or start your free trial to access this content
This manuscript details the fabrication of micro-tissue engineered neural networks: three-dimensional micron-sized constructs comprised of long aligned axonal tracts spanning aggregated neuronal population(s) encased in a tubular hydrogel. These living scaffolds can serve as functional relays to reconstruct or modulate neural circuitry or as biofidelic test-beds mimicking gray-white matter neuroanatomy.