In Vivo VESC Vessel Formation Assay Using the Mammay Fat Pad
8:05
Whole Mount Tissue Preparation for Microscopic Observation
10:23
Results: Isolation of Vascular Endothelial Stem Cells and Vessel Generation of Tranplanted Cells
11:27
Conclusion
Transkript
The overall goal of this experiment is to assess the proliferation, differentiation, and vessel generation potential of vascular endothelial stem cells through a mammary fat pad transplantation technique followed by whole-mount tissue preparation
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This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.