Most Probable Number (MPN) Conjugation Frequency Calculation
2:37
Results: Representative Probability of Donor-Initiated Conjugation Between Plasmids
5:01
Conclusion
Transkript
This method can help answer key questions in the microbiology field about how often plasmid DNA can be spread among different bacteria. The main advantage of this technique is that by reducing the background, we can detect small differences in the
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With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.