<meta charset="utf8"></head><body> Herstellung von epimysialen EMG-Elektroden für präklinische Nagetiermodelle

<ol>
	<li>Grandjean, P. A., Mortimer, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recruitment+properties+of+monopolar+and+bipolar+epimysial+electrodes.">Recruitment properties of monopolar and bipolar epimysial electrodes.</a> Ann. Biomed. Eng. 14 (1), 53-66 (1986).</li><li>Memberg, W. D., Stage, T. G., Kirsch, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+fully+implanted+intramuscular+bipolar+myoelectric+signal+recording+electrode.">A fully implanted intramuscular bipolar myoelectric signal recording electrode.</a> Neuromodulation J. Int. Neuromodulation Soc. 17 (8), 794-799 (2014).</li><li>Loeb, G. E., Gans, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electromyography for Experimentalists. , (1986).</li><li>Boehler, C., Carli, S., Fadiga, L., Stieglitz, T., Asplund, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tutorial:+guidelines+for+standardized+performance+tests+for+electrodes+intended+for+neural+interfaces+and+bioelectronics.">Tutorial: guidelines for standardized performance tests for electrodes intended for neural interfaces and bioelectronics.</a> Nat. Protoc. 15 (11), 3557-3578 (2020).</li><li>Patel, P. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Insertion+of+linear+8.4+&#181;m+diameter+16+channel+carbon+fiber+electrode+arrays+for+single+unit+recordings.">Insertion of linear 8.4 &#181;m diameter 16 channel carbon fiber electrode arrays for single unit recordings.</a> J. Neural Eng. 12 (4), 046009 (2015).</li><li>Richie, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Open-source+toolkit:+benchtop+carbon+fiber+microelectrode+array+for+nerve+recording.">Open-source toolkit: benchtop carbon fiber microelectrode array for nerve recording.</a> J. Vis. Exp. (176), e63099 (2021).</li><li>Sarolic, A., Skalic, I., Deftu, A., Sapunar, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impedance+measurement+of+bipolar+stimulation+electrodes+immersed+in+medium.">Impedance measurement of bipolar stimulation electrodes immersed in medium.</a> , 1-2 (2018).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+rodent+surgery+for+the+New+Surgeon.">Principles of rodent surgery for the New Surgeon.</a> J. Vis. Exp.: JoVE. (47), e2586 (2011).</li><li>Zealear, D., Li, Y., Huang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+implantable+system+for+chronic+in+vivo+electromyography.">An implantable system for chronic in vivo electromyography.</a> J. Vis. Exp. JoVE. (158), e60345 (2020).</li><li>Butterfield, T. A., Leonard, T. R., Herzog, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+serial+sarcomere+number+adaptations+in+knee+extensor+muscles+of+rats+is+contraction+type+dependent.">Differential serial sarcomere number adaptations in knee extensor muscles of rats is contraction type dependent.</a> J. Appl. Physiol. Bethesda Md 1985. 99 (4), 1352-1358 (2005).</li><li>Farago, E., MacIsaac, D., Suk, M., Chan, A. D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+techniques+for+surface+electromyography+signal+quality+analysis.">A review of techniques for surface electromyography signal quality analysis.</a> IEEE Rev. Biomed. Eng. 16, 472-486 (2023).</li><li>Raez, M. B. I., Hussain, M. S., Mohd-Yasin, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+of+EMG+signal+analysis:+detection,+processing,+classification+and+applications.">Techniques of EMG signal analysis: detection, processing, classification and applications.</a> Biol. Proced. Online. 8, 11-35 (2006).</li><li>Tankisi, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Standards+of+instrumentation+of+EMG.">Standards of instrumentation of EMG.</a> Clin. Neurophysiol. 131 (1), 243-258 (2020).</li><li>Kumar, A., Accorsi, A., Younghwa, R., Mahasweta, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Do's+and+don'ts+in+the+preparation+of+muscle+cryosections+for+histological+analysis.">Do's and don'ts in the preparation of muscle cryosections for histological analysis.</a> J. Vis. Exp. JoVE. (99), e52793 (2015).</li><li>Wang, C., Yue, F., Kuang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Muscle+histology+characterization+using+H&amp;E+staining+and+muscle+fiber+type+classification+using+immunofluorescence+staining.">Muscle histology characterization using H&amp;E staining and muscle fiber type classification using immunofluorescence staining.</a> Bio-Protoc. 7 (10), e2279 (2017).</li><li>Kreifeldt, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Signal+versus+noise+characteristics+of+filtered+EMG+used+as+a+control+source.">Signal versus noise characteristics of filtered EMG used as a control source.</a> IEEE Trans. Biomed. Eng. BME-18 (1), 16-22 (1971).</li><li>Farina, D., Yoshida, K., Stieglitz, T., Koch, K. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multichannel+thin-film+electrode+for+intramuscular+electromyographic+recordings.">Multichannel thin-film electrode for intramuscular electromyographic recordings.</a> J. Appl. Physiol. 104 (3), 821-827 (2008).</li><li>Muceli, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decoding+motor+neuron+activity+from+epimysial+thin-film+electrode+recordings+following+targeted+muscle+reinnervation.">Decoding motor neuron activity from epimysial thin-film electrode recordings following targeted muscle reinnervation.</a> J. Neural Eng. 16 (1), 016010 (2018).</li><li>Guo, L., Guvanasen, G., Tuthill, C., Nichols, T., Deweerth, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Characterization of a Stretchable Multielectrode Array for Epimysial Recording. , 694 (2011).</li><li>Zwarts, M. J., Stegeman, D. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multichannel+surface+EMG:+basic+aspects+and+clinical+utility.">Multichannel surface EMG: basic aspects and clinical utility.</a> Muscle Nerve. 28 (1), 1-17 (2003).</li><li>Koch, K. P., Leinenbach, C., Stieglitz, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+and+test+of+robust+spherical+epimysial+electrodes+for+lower+limb+stimulation.">Fabrication and test of robust spherical epimysial electrodes for lower limb stimulation.</a> Aalb. Den. , (2000).</li><li>Uhlir, J. P., Triolo, R. J., Davis, J. A., Bieri, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Performance+of+epimysial+stimulating+electrodes+in+the+lower+extremities+of+individuals+with+spinal+cord+injury.">Performance of epimysial stimulating electrodes in the lower extremities of individuals with spinal cord injury.</a> IEEE Trans. Neural Syst. Rehabil. Eng. 12 (2), 279-287 (2004).</li><li>Deer, T. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+appropriate+use+of+neurostimulation:+new+and+evolving+neurostimulation+therapies+and+applicable+treatment+for+chronic+pain+and+selected+disease+states.">The appropriate use of neurostimulation: new and evolving neurostimulation therapies and applicable treatment for chronic pain and selected disease states.</a> Neuromodulation Technol. Neural Interface. 17 (6), 599-615 (2014).</li><li>Ortiz-Catalan, M., Br&#229;nemark, R., H&#229;kansson, B., Delbeke, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+viability+of+implantable+electrodes+for+the+natural+control+of+artificial+limbs:+Review+and+discussion.">On the viability of implantable electrodes for the natural control of artificial limbs: Review and discussion.</a> Biomed. Eng. OnLine. 11, 33 (2012).</li><li>Sando, I. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regenerative+peripheral+nerve+interface+for+prostheses+control:+electrode+comparison.">Regenerative peripheral nerve interface for prostheses control: electrode comparison.</a> J. Reconstr. Microsurg. 32 (3), 194-199 (2016).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Electrode Materials</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>Quantity &#38; price per electrode</td>
		</tr>
		<tr>
			<td>Contact surface</td>
			<td>Prince and Izant PT90/IR10 1.25 mm x 5 mm foil</td>
			<td>Catalog #1040055&#160;</td>
			<td>2 per electrode 
			$7.50 per foil 
			$15.00 per electrode</td>
		</tr>
		<tr>
			<td>PFA coated stainless-steel electrode lead wire&#160;</td>
			<td>A-M Systems Multi-Stranded PFA-Coated Stainless Steel Wire 50.8 &#181;m strand diameter&#160;</td>
			<td>Catalog #793500</td>
			<td>Dependent on desired lead length (e.g., 9 inch lead wires x2) 
			$128 per 25 ft spool 
			$5.12 per foot 
			$0.42 per inch (x18) 
			$7.68 per electrode</td>
		</tr>
		<tr>
			<td>Folding jig&#160;</td>
			<td>3D printed 
			(see .gcode file)</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Sealant for electrode body</td>
			<td>Nusil Med-1137 liquid silicone</td>
			<td>Catalog #MED-1137</td>
			<td>1 gram 
			$344.66 per 2 oz. (59.15 mL) 
			$5.83 per electrode</td>
		</tr>
		<tr>
			<td>Silicone base</td>
			<td>Implantech Alliedsil Silicone Sheeting-Reinforced, Long Term Implantable (8&#8221; x 6&#8221;) .007 thick</td>
			<td>Catalog #701-07&#160;</td>
			<td>10mm x 5mm sheet 
			$225.00 per 8 x 6 inch 
			$0.36 per electrode (10 mm x 5 mm)</td>
		</tr>
		<tr>
			<td>&#160;Thinner for sealant mixture</td>
			<td>Toluene 99.5% ACS Reagent 500mL or Xylene ACS 99.5%</td>
			<td>Catalog #179418-500 ML</td>
			<td>0.75 mL 
			$25.53 per 500 mL 
			$0.38 per electrode</td>
		</tr>
		<tr>
			<td>Template for perforating silicone base</td>
			<td>Cutting jig &#8211; 3D printed 
			(see CAD file)</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Custom-fabricated electrode: $29.25</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Industry standard electrode (EP105 EMG Patch Electrode, 2 contacts, single-sided, 7mm x 4mm, MicroProbe for Life Science): $305.00</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Additional Fabrication Materials&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>&#160; &#160;&#160;</td>
			<td>&#160; &#160;&#160;</td>
			<td>&#160; &#160;&#160;</td>
			<td>Quantity &#38; price per electrode</td>
		</tr>
		<tr>
			<td>3D printing software&#160;</td>
			<td>Solidworks (Solidworks, 2022)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Micro-Tig welder&#160;</td>
			<td>Micro-Tig Welder (CD1000SPM, Single Pulse Research and Light Production Resistance Spot Welder, Sunstone)</td>
			<td>SKU&#160;301010</td>
			<td>$3,500</td>
		</tr>
		<tr>
			<td>Ultrasonic bath</td>
			<td>Ultrasonic bath (CPX Series Ultrasonic Bath, Fisherbrand).&#160;</td>
			<td>15-337-403</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Ex Vivo Testing Materials&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>Quantity &#38; price per electrode</td>
		</tr>
		<tr>
			<td>Data acquisition platform and software</td>
			<td>DigitalLynx 4sX Base Cheetah version 6.0 (Neuralynx Inc.)&#160;</td>
			<td>NA</td>
			<td>EMG acquisition hardware and software</td>
		</tr>
		<tr>
			<td>Electrode interface board (EIB)</td>
			<td>EIB, EIB16-QC, Neuralynx Inc.</td>
			<td>31-0603-0007</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Signal generator</td>
			<td>5 MHz Function Generator, B&#38;K Precision&#160;</td>
			<td>&#160;4005DDS220V</td>
			<td>$387.46</td>
		</tr>
		<tr>
			<td>Potentiostat</td>
			<td>PGSTAT1 potentiostat (EcoChemie, Utrecht, Netherlands)</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Stainless steel screw</td>
			<td>Fine Science Tools</td>
			<td>19010-00</td>
			<td>$98</td>
		</tr>
		<tr>
			<td>Ex Vivo Testing Materials&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>Quantity &#38; price per electrode</td>
		</tr>
		<tr>
			<td>Rodent treadmill&#160;</td>
			<td>Exer 3/6 Open Treadmill, Columbus Instruments</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Dental cement</td>
			<td>Excel Formula&#174; Pourable Dental Material, St. George Technology Inc.</td>
			<td>#24211</td>
			<td>$125.60</td>
		</tr>
		<tr>
			<td>Light microscope</td>
			<td>Keyence BZ-X800, Keyence Corporation, Osaka, Japan&#160;</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Motion capture system</td>
			<td>Optitrack Color Camera, Optitrack, NaturalPoint Inc.</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Peak detection algorithm</td>
			<td>&#8220;SciPy.signal.find_peaks - SciPy v1.8.1 Manual&#8221;, 2022</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Python software</td>
			<td>Python Software Foundation. Python Language Reference, version 3.9. Available at http://www.python.org</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Rat</td>
			<td>HsdBlu: LE, Envigo</td>
			<td>140</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Statistical sotware</td>
			<td>GraphPad Prism version 10.0.0 (GraphPad Software, Boston, Massachusetts USA)</td>
			<td>NA</td>
			<td>NA</td>
		</tr>
	</tbody>
</table>

development of a low-cost epimysial electromyography electrode: a simplified workflow for fabrication and testing

Electromyography (EMG) is a powerful tool for studying the electrical activity of muscle. EMG recordings can be especially useful in preclinical animal models to assess the effectiveness of interventions to treat neuromuscular dysfunction. In these models, implantable biocompatible electrodes are commonly used to assess the neurophysiological interface between motor neurons and muscle fibers. These implantable electrodes can provide localized measurements of muscle excitation and can be diverse in terms of their configuration, shape, and material, with the optimal design ultimately dictated by the location and intended use.
Despite their suitability for assessing muscle excitation in preclinical models, the use of epimysial electrodes can be limited by cost. As a result, many investigators use custom-fabricated epimysial electrodes that are produced in-house. Although resources exist detailing the fundamental considerations of electrode fabrication, testing, and use1,2, there is a need for an updated instructional guide detailing the sourcing, fabrication, and validation of epimysial electrodes using modern methods. Informed by the foundational works of Loeb and Gans3 and others in electrode theory, we present modern instructions on the sourcing and fabrication of low-cost epimysial electrodes and test their performance in a series of ex vivo and in vivo experiments. The aim is to offer a user-friendly guide for others in the scientific community to source, fabricate, and test in-house low-cost epimysial electrodes for animal use, enabling the broader quantification of muscle excitation in preclinical models.
In this protocol, we provide an instructional guide to the sourcing, fabrication, and testing of epimysial electrodes for animal use in the modern electrophysiology laboratory. Electrode parameters chosen for fabrication, such as the shape, dimensions, contact surface area, interelectrode distance, lead length, etc., were selected to suit our experimental needs and were comparable to a commercially available industry standard epimysial electrode (see the Table of Materials). We encourage other groups to modify these parameters to suit their needs in addition to selecting a reliable industry standard electrode that matches their use case.
In an effort to give readers a relatively quick sense of electrode performance, we also provide an example of an ex vivo testing protocol with the option of measuring electrode impedance. Additionally, we give an example assessment of electrode performance in vivo. The ex vivo experiment compared the custom-fabricated electrode to an industry standard in a saline bath to mimic stable physiological conditions. Impedance was also assessed ex vivo via electrochemical impedance spectroscopy (EIS). The in vivo experiment consisted of the surgical implantation of the custom-fabricated electrode into the vastus lateralis (VL) muscle of a 16-week-old female Long Evans rat (HsdBlu: LE, Envigo) to measure the EMG signal during conditions known to elicit a high or low signal (uphill, downhill walking). To assess the reliability of the custom-fabricated electrode, EMG signaling was acquired during level walking following full surgical recovery and prior to sacrifice (14 days and 56 days post implantation, respectively). Hematoxylin-eosin (H&#38;E) staining was conducted on the instrumented muscle to assess the biocompatibility of the custom-fabricated electrode.

<meta charset="utf8"></head><body>Autor im Rampenlicht: Herstellung und Prüfung epimysieller Elektroden in Studien zu ACL-Verletzungen

Entwicklung einer kostengünstigen epimysialen Elektromyographie-Elektrode: Ein vereinfachter Arbeitsablauf für Herstellung und Prüfung

Read this Scientific Article Protocol about Author Spotlight: Epimysial Electrode Fabrication and Testing in ACL Injury Studies at JoVE.com

Electromyography (EMG) is a valuable diagnostic tool for detecting neuromuscular abnormalities. Implantable epimysial electrodes are commonly used to ...

development-low-cost-epimysial-electromyography-electrode-simplified

Research

JoVE Journal

Neuroscience

Development of a Low-cost Epimysial Electromyography Electrode: A Simplified Workflow for Fabrication and Testing

277 Aufrufe.  University of Michigan, Ann Arbor. Wir versuchen, die neuromuskulären und elektrisch-physiologischen Folgen einer VKB-Verletzung anhand eines präklinischen Nagetiermodells zu verstehen. In unseren aktuellen Experimenten untersuchen wir mit implantierbaren epimysialen EMG-Elektroden, wie eine nicht-invasive VKB-Verletzung die Quadrizepsaktivierung verändert. Obwohl es grundlegende Ressourcen gibt, in denen die Überlegungen zur Elektrodenherstellung und -prüfung detailliert beschrieben werd...

Serie: Autor im Rampenlicht: Herstellung und Prüfung epimysieller Elektroden in Studien zu ACL-Verletzungen

Electromyography (EMG) is a valuable diagnostic tool for detecting neuromuscular abnormalities. Implantable epimysial electrodes are commonly used to measure EMG signals in preclinical models. Although classical resources exist describing the principles of epimysial electrode fabrication, there is a sparsity of illustrative information translating electrode theory to practice. To remedy this, we provide an updated, easy-to-follow guide on fabricating and testing a low-cost epimysial electrode. 
Electrodes were made by folding and inserting two platinum-iridium foils into a precut silicone base to form the contact surfaces. Next, coated stainless steel wires were welded to each contact surface to form the electrode leads. Lastly, a silicone mixture was used to seal the electrode. Ex vivo testing was conducted to compare our custom-fabricated electrode to an industry standard electrode in a saline bath, where high levels of signal agreement (sine [intraclass correlation - ICC= 0.993], square [ICC = 0.995], triangle [ICC = 0.958]), and temporal-synchrony (sine [r = 0.987], square [r = 0.990], triangle [r= 0.931]) were found across all waveforms. Low levels of electrode impedance were also quantified via electrochemical impedance spectroscopy. 
An in vivo performance assessment was also conducted where the vastus lateralis muscle of a rat was surgically instrumented with the custom-fabricated electrode and signaling was acquired during uphill and downhill walking. As expected, peak EMG activity was significantly lower during downhill walking (0.008 &plusmn; 0.005 mV) than uphill (0.031 &plusmn; 0.180 mV, p = 0.005), supporting the validity of the device. The reliability and biocompatibility of the device were also supported by consistent signaling during level walking at 14 days and 56 days post implantation (0.01 &plusmn; 0.007 mV, 0.012 &plusmn; 0.007 mV respectively; p &gt; 0.05) and the absence of histological inflammation. Collectively, we provide an updated workflow for the fabrication and testing of low-cost epimysial electrodes.

Our purpose was to provide an updated, easy-to-follow guide on the fabrication and testing of epimysial electromyography electrodes. To that end, we provide instructions for material sourcing and a detailed walkthrough of the fabrication and testing process.