basic biology

Basic Biology

advanced biology

Advanced Biology

clinical skills

Clinical Skills

chemistry

Chemistry

environmental sciences

Environmental Sciences

physics

Physics

engineering

Engineering

psychology

Psychology

jove science education

JoVE Science Education

A video library dedicated to teaching the practice and theory of scientific experiments through engaging and easy-to-understand visual demonstrations.

Education

using a hemacytometer to count cells

Using a Hemacytometer to Count Cells

Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant ...

passaging cells

Passaging Cells

Cell lines are frequently used in biomedical experiments, as they allow rapid culture and expansion of cell types for experimental analysis. Cell lines ...

pcr: the polymerase chain reaction

PCR: The Polymerase Chain Reaction

The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling &#8211; cyles of temperature changes at fixed time ...

dna gel electrophoresis

DNA Gel Electrophoresis

DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of ...

separating protein with sds-page

Separating Protein with SDS-PAGE

Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis, or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their ...

bacterial transformation: the heat shock method

bacterial-transformation-the-heat-shock-method

Bacterial Transformation: The Heat Shock Method

Transformation is the process that occurs when a cell ingests foreign DNA from its surroundings. Transformation can occur in nature in certain types of ...

bacterial transformation: electroporation

Bacterial Transformation: Electroporation

The term &#x201C;transformation&#x201D; refers cellular ingestion of foreign DNA.  In nature, transformation can occur in certain types of bacteria.  In ...

the elisa method

The ELISA Method

An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an ...

plasmid purification

Plasmid Purification

Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid ...

gel purification

Gel Purification

Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, ...

the western blot

The Western Blot

Western Blotting is used to identify the presence of specific proteins in electrophoretically separated samples.  Following separation by a technique ...

an introduction to transfection

An Introduction to Transfection

Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell ...

dna ligation reactions

DNA Ligation Reactions

In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond.  An enzyme known as a ligase ...

restriction enzyme digests

Restriction Enzyme Digests

Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence.  These enzymes occur naturally in bacteria as a defense against ...

molecular cloning

Molecular Cloning

Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant ...

basic methods in cellular and molecular biology

Basic Methods in Cellular and Molecular Biology

This collection demonstrates how to execute basic techniques commonly used in cellular and molecular biology.