expression-recombinant-cellulase-cel5a-from-trichoderma-reesei

Expression of Recombinant Cellulase Cel5A from <em>Trichoderma reesei</em> in Tobacco Plants

Cellulose degrading enzymes, cellulases, are targets of both research and industrial interests. The preponderance of these enzymes in difficult-to-culture ...

fractionation-of-lignocellulosic-biomass-using-the-organocat-process

Fractionation of Lignocellulosic Biomass using the OrganoCat Process

The shift from a petroleum-based to a more sustainable and bio-based economy requires the development of new refinery concepts to maintain the supply of ...

RWTH Aachen University

Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich

The study was conducted to evaluate the long-term results of homograft reconstruction of the left ventricular outflow tract with a cryopreserved aortic homograft in the presence of aortic root abscess associated with a biofilm bacterial infection.

Homograft reconstruction of the aortic root for endocarditis with periannular abscess: a 17-year study.

We retrospectively analyzed the outcome of heart valve operations in solid organ recipients, who were referred for operation to our institution.

Heart valve operations in solid organ recipients: an 18-year single-center experience.

Hyperthermophilic endoglucanase for in planta lignocellulose conversion.

Plant-produced biomass-degrading enzymes are promising tools for the processing of lignocellulose to fermentable sugars. A major limitation of in planta production is that high-level expression of such enzymes could potentially affect the structure and integrity of the plant cell wall and negatively influence plant growth and development.

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development.

Improvement of cellulase expression has the potential to change the nature of the biofuel industry. Increasing the economic feasibility of cellulase systems would significantly broaden the range of practicable biomass conversion, lowering the environmental impact of our civilisations' fuel needs. Cellulases are derived from certain fungi and bacteria, which are often difficult to culture on an industrial scale. Accordingly, methods to recombinantly express important cellulases and other glycosyl hydrolase (GH) enzymes are under serious investigation. Herein, we examine the latest developments in bacterial, yeast, plant, and fungal expression systems. We discuss current strategies for producing cellulases, and evaluate the benefits and drawbacks in yield, stability, and activity of enzymes from each system, and the overall progress in the field.

Cellulases for biomass degradation: comparing recombinant cellulase expression platforms.

To develop novel biorefinery concepts, the use of bio-based catalysts and solvents must be aligned with the principles of green chemistry. In this context, biogenic 2,5-furandicarboxylic acid (FDCA) is a very promising yet underused molecule with high potential for application as an acid catalyst, combining feasibility and sustainability with efficient and straightforward recovery. In this study, FDCA was evaluated as a catalyst in the recently developed OrganoCat pretreatment, a biphasic lignocellulose fractionation system. The catalyst was investigated for the efficient fractionation of the three main components-lignin, cellulose and noncellulosic sugars-with particular focus on the lignin quality, on the effect on enzymatic hydrolysis of the cellulosic residue, and on the noncellulosic sugar extraction. To address recovery of FDCA from the OrganoCat system, a method was developed, leading to the recovery of >97 % of FDCA with a spectroscopic purity of >99 %, maintaining full activity in consecutive runs.

Article Total : 2	Year
Publication title	2014
Publication title	2021

Article	Year
Homograft reconstruction of the aortic root for endocarditis with periannular abscess: a 17-year study. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery\| PubMed ID: 15982588	2005
Heart valve operations in solid organ recipients: an 18-year single-center experience. Transplantation\| PubMed ID: 17876271	2007
Hyperthermophilic endoglucanase for in planta lignocellulose conversion. Biotechnology for biofuels\| PubMed ID: 22928996	2012
Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development. Biotechnology for biofuels\| PubMed ID: 23587418	2013
Cellulases for biomass degradation: comparing recombinant cellulase expression platforms. Trends in biotechnology\| PubMed ID: 23910542	2013
One-Step Lignocellulose Fractionation by using 2,5-Furandicarboxylic Acid as a Biogenic and Recyclable Catalyst. ChemSusChem\| PubMed ID: 29791080	2018

Holger Klose

Biography

JoVE Journal Publications

Other Publications