Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich

2 ARTICLES PUBLISHED IN JoVE

Environment

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Megan Garvey¹, Johannes Klinger^*1, Holger Klose^*1, Rainer Fischer², Ulrich Commandeur¹

¹Institute for Molecular Biotechnology, Bio7, RWTH Aachen University, ²Fraunhofer Institute for Molecular Biology and Applied Ecology

Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

Chemistry

Fractionation of Lignocellulosic Biomass using the OrganoCat Process

Leonie Schoofs¹, Dennis Weidener¹, Ulrich Schurr¹, Holger Klose¹, Philipp M. Grande¹

¹Institut für Bio- und Geowissenschaften, Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich

OrganoCat is a method for the pretreatment and fractionation of lignocellulose under mild conditions into lignin, fermentable sugars, and cellulose pulp. In a biogenic, biphasic solvent system of water and 2-methyltetrahydrofuran with 2,5-furancarboxylic acid as catalyst, the OrganoCat products are separated in situ for straightforward product recovery.