electrophysiological-investigations-retinogeniculate

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

The lateral geniculate nucleus is the first relay station for the visual information. Relay neurons of this thalamic nucleus integrate input from retinal ...

University Medical Center of the Johannes Gutenberg University Mainz

Relay neurons in the dorsal lateral geniculate nucleus (dLGN) receive excitatory inputs from retinal ganglion cells (RGCs). Retinogeniculate synapses are characterized by a prominent short-term depression of AMPA receptor (AMPAR)-mediated currents, but the underlying mechanisms and its function for visual integration are not known. Here we identify CKAMP44 as a crucial auxiliary subunit of AMPARs in dLGN relay neurons, where it increases AMPAR-mediated current amplitudes and modulates gating of AMPARs. Importantly, CKAMP44 is responsible for the distinctive short-term depression in retinogeniculate synapses by reducing the rate of recovery from desensitization of AMPARs. Genetic deletion of CKAMP44 strongly reduces synaptic short-term depression, which leads to increased spike probability of relay neurons when activated with high-frequency inputs from retinogeniculate synapses. Finally, in vivo recordings reveal augmented ON- and OFF-responses of dLGN neurons in CKAMP44 knockout (CKAMP44) mice, demonstrating the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration.

Xufeng Chen

Institute of Pathophysiology

University Medical Center of the Johannes Gutenberg University Mainz

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

Xufeng Chen

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Institute of Pathophysiology

University Medical Center of the Johannes Gutenberg University Mainz

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

Institute of Pathophysiology