microfluidic-synthesis-microgel-building-blocks-for-microporous

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

The microporous annealed particle (MAP) scaffold platform is a subclass of granular hydrogels. It is composed of an injectable slurry of microgels that ...

quantifying-three-dimensional-cell-migration-within-into-granular

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Granular hydrogel scaffolds hold significant potential in regenerative medicine, functioning either as carriers for cell delivery or as interfaces for ...

University of Virginia

Thiol-norbornene photoclick hydrogels are highly efficient in tissue engineering applications due to their fast gelation, cytocompatibility, and tunability. In this work, we utilized the advantageous features of polyethylene glycol (PEG)-thiol-ene resins to enable fabrication of complex and heterogeneous tissue scaffolds using 3D bioprinting and in-air drop encapsulation techniques. We demonstrated that photoclickable PEG-thiol-ene resins could be tuned by varying the ratio of PEG-dithiol to PEG norbornene to generate a wide range of mechanical stiffness (0.5-12 kPa) and swelling ratios. Importantly, all formulations maintained a constant, rapid gelation time (<0.5 s). We used this resin in biological projection microstereolithography (BioPSL) to print complex structures with geometric fidelity and demonstrated biocompatibility by printing cell-laden microgrids. Moreover, the rapid gelling kinetics of this resin permitted high-throughput fabrication of tunable, cell-laden microgels in air using a biological in-air drop encapsulation apparatus (BioIDEA). We demonstrated that these microgels could support cell viability and be assembled into a gradient structure. This PEG-thiol-ene resin, along with BioPSL and BioIDEA technology, will allow rapid fabrication of complex and heterogeneous tissues that mimic native tissues with cellular and mechanical gradients. The engineered tissue scaffolds with a controlled microscale porosity could be utilized in applications including gradient tissue engineering, biosensing, andtissue models.

Juliana Trujillo

Department of Biomedical Engineering,
School of Engineering and Applied Sciences,
Department of Biomedical Engineering, School of Engineering and Applied Sciences

University of Virginia

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Juliana Trujillo

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Biomedical Engineering,School of Engineering and Applied Sciences,Department of Biomedical Engineering, School of Engineering and Applied Sciences

University of Virginia

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Department of Biomedical Engineering,
School of Engineering and Applied Sciences,
Department of Biomedical Engineering, School of Engineering and Applied Sciences