Zhilei Chen

Directed evolution of enzymes and pathways for industrial biocatalysis.

A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors.

Directed evolution of human estrogen receptor variants with significantly enhanced androgen specificity and affinity.

Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches.

Rapid creation of a novel protein function by in vitro coevolution.

A highly sensitive selection method for directed evolution of homing endonucleases.

Directed evolution of homing endonuclease I-SceI with altered sequence specificity.

A cell protection screen reveals potent inhibitors of multiple stages of the hepatitis C virus life cycle.

Creation and characterization of a cell-death reporter cell line for hepatitis C virus infection.

PD 404,182 is a virocidal small molecule that disrupts hepatitis C virus and human immunodeficiency virus.

Probing the role of sigma π interaction and energetics in the catalytic efficiency of endo-1,4-β-xylanase.

Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.

Intein-triggered artificial protein hydrogels that support the immobilization of bioactive proteins.

Phenothiazines inhibit hepatitis C virus entry, likely by increasing the fluidity of cholesterol-rich membranes.

Split intein mediated ultra-rapid purification of tagless protein (SIRP).

Characterization of an acid-tolerant β-1,4-glucosidase from Fusarium oxysporum and its potential as an animal feed additive.

Two-component protein hydrogels assembled using an engineered disulfide-forming protein-ligand pair.

Discovery and characterization of a new cell-penetrating protein.

Evaluation of PD 404,182 as an anti-HIV and anti-HSV microbicide.

A screen for genetic suppressor elements of hepatitis C virus identifies a supercharged protein inhibitor of viral replication.