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Yamaguchi University of Medicine

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Kinya Matsuo¹, Britta Engelhardt², Hideaki Nishihara³

¹Graduate School of Medicine, Yamaguchi University, ²Theodor Kocher Institute, University of Bern, ³Department of Neurotherapeutics, Yamaguchi University of Medicine

Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.