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UK Dementia Research Institute

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice

Mark Gurney¹, Luke C. Davies^1,2, Ruth E. Jones¹, Valentina M. Bart¹, Robert H. Jenkins¹, Paul Brennan¹, Philip R. Taylor^1,3, Magdalena A. Czubala¹

¹Systems Immunity Research Institute, Division of Infection and Immunity, Cardiff University, ²Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science, Swansea University, ³UK Dementia Research Institute

We demonstrate a step-by-step protocol for the investigation of gene function in peritoneal tissue-resident macrophages in vivo, using lentiviral vectors.