Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in MiceMark Gurney 1, Luke C. Davies 1,2, Ruth E. Jones 1, Valentina M. Bart 1, Robert H. Jenkins 1, Paul Brennan 1, Philip R. Taylor 1,3, Magdalena A. Czubala 1
1Systems Immunity Research Institute, Division of Infection and Immunity, Cardiff University, 2Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science, Swansea University, 3UK Dementia Research Institute
We demonstrate a step-by-step protocol for the investigation of gene function in peritoneal tissue-resident macrophages in vivo, using lentiviral vectors.
