CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid LibrarySi Xu *1,2, Mengyu Chen *1,2, Guang Chen 1,3, Si Luo 4, Wen Xue 1,2, Xuelian Liu 1,2, Jiwu Wang 1,2,3, Ping Wei 4
1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
We present a protocol for CRISPR-based modular assembly (CRISPRmass), a method for high-throughput construction of UAS-cDNA/ORF plasmid library in Drosophila using publicly available cDNA/ORF resources. CRISPRmass can be applied to editing various plasmid libraries.
