Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion CellsJialiang Yang *1, Jiaxin Guo *1, Jing Hu *1,2, Xiawei Wu *1, Haotian Huang 1, Yu Wen 1, Runfang Chen 1, Congrui Liu 1, Houbin Zhang 1,3
1Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 2School of Medicine, University of Electronic Science and Technology of China, 3Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
This protocol describes the procedure for isolating the whole-mount mouse retina and performing immunostaining to label all retinal ganglion cells (RGCs). The process is followed by imaging and automatically counting RGCs using AI-based software, providing a simple, fast, and accurate method for quantifying RGCs in the entire mouse retina.
