Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion Cells

Jialiang Yang^*1, Jiaxin Guo^*1, Jing Hu^*1,2, Xiawei Wu^*1, Haotian Huang¹, Yu Wen¹, Runfang Chen¹, Congrui Liu¹, Houbin Zhang^1,3

¹Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ²School of Medicine, University of Electronic Science and Technology of China, ³Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

This protocol describes the procedure for isolating the whole-mount mouse retina and performing immunostaining to label all retinal ganglion cells (RGCs). The process is followed by imaging and automatically counting RGCs using AI-based software, providing a simple, fast, and accurate method for quantifying RGCs in the entire mouse retina.

Neuroscience

Cryosectioning and Immunostaining of Mouse Retina

Yongqiong Lin^*1, Yingjie Tong^*1, Tongdan Zou¹, Jiajia Wang¹, Houbin Zhang^1,2

¹The Key Laboratory for Human Disease Gene Study of Sichuan Province and Department of Laboratory Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, ²Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

A protocol for preparing mouse retinal cryosections and performing immunostaining on photoreceptors is described. This article enables researchers to consistently produce mouse retinal frozen sections with well-preserved morphology and high-quality immunostaining results.