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Radboud university medical centre

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Xuehui He¹, Vivian L. de Oliveira¹, Romy Keijsers², Irma Joosten¹, Hans JPN Koenen¹

¹Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Centre, ²Department of Dermatology, Radboud University Medical Centre

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

JoVE Core

Management of Respiratory Motion Artefacts in ¹⁸F-fluorodeoxyglucose Positron Emission Tomography using an Amplitude-Based Optimal Respiratory Gating Algorithm

Willem Grootjans¹, Peter Kok², Jurrian Butter², Erik Aarntzen²

¹Department of Radiology, Leiden University Medical Centre, ²Department of Radiology and Nuclear Medicine, Radboud University Medical Centre

Amplitude-based optimal respiratory gating (ORG) effectively removes respiratory-induced motion blurring from clinical ¹⁸F-fluorodeoxyglucose (FDG) positron emission tomography (PET) images. Correction of FDG-PET images for these respiratory motion artefacts improves image quality, diagnostic and quantitative accuracy. Removal of respiratory motion artefacts is important for adequate clinical management of patients using PET.