Radboud university medical centre
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Xuehui He 1, Vivian L. de Oliveira 1, Romy Keijsers 2, Irma Joosten 1, Hans JPN Koenen 1
1Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Centre, 2Department of Dermatology, Radboud University Medical Centre
We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.
JoVE Core
Management of Respiratory Motion Artefacts in 18F-fluorodeoxyglucose Positron Emission Tomography using an Amplitude-Based Optimal Respiratory Gating Algorithm
Willem Grootjans 1, Peter Kok 2, Jurrian Butter 2, Erik Aarntzen 2
1Department of Radiology, Leiden University Medical Centre, 2Department of Radiology and Nuclear Medicine, Radboud University Medical Centre
Amplitude-based optimal respiratory gating (ORG) effectively removes respiratory-induced motion blurring from clinical 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) images. Correction of FDG-PET images for these respiratory motion artefacts improves image quality, diagnostic and quantitative accuracy. Removal of respiratory motion artefacts is important for adequate clinical management of patients using PET.
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