Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for BeginnersBrian Dinh 1, Marten A. Hoeksema 2, Nathanael J. Spann 3, Jacob Rendler 1, Isidoro Cobo 4,5, Christopher K. Glass 3,6, Calvin Yeang 1
1Division of Cardiology, Department of Medicine, University of California San Diego, 2Department of Medical Biochemistry, Amsterdam Immunity and Infection: Inflammatory diseases; Amsterdam Cardiovascular Sciences: Atherosclerosis and Ischemic Syndrome, Amsterdam UMC location University of Amsterdam, 3Department of Cellular and Molecular Medicine, University of California San Diego, 4Division of Clinical Immunology & Rheumatology, Department of Medicine, Heersink School of Medicine, University of Alabama at Birmingham, 5Comprehensive Arthritis, Musculoskeletal, Bone and Autoimmunity Center, Heersink School of Medicine, University of Alabama at Birmingham, 6Department of Medicine, University of California San Diego
This protocol presents an accessible guide for collecting, storing, and thawing peripheral blood mononuclear cells suitable for downstream analyses and workflows like flow cytometry and RNA sequencing. Plasma and buffy coat collections are also demonstrated.
