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Food and Drug Administration

4 ARTICLES PUBLISHED IN JoVE

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Biology

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Baoguang Li 1, Zonglin Hu 1, Christopher A. Elkins 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples

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Behavior

Barnes Maze Testing Strategies with Small and Large Rodent Models
Cheryl S. Rosenfeld *1, Sherry A. Ferguson *2
1Biomedical Sciences and Bond Life Sciences Center, University of Missouri, 2Division of Neurotoxicology, National Center for Toxicological Research, Food and Drug Administration

The dry-land Barnes maze is widely used to measure spatial navigation ability in response to mildly aversive stimuli. Over consecutive days, performance (e.g. latency to locate escape cage) of control subjects improves, indicative of normal learning and memory. Differences between rats and mice necessitate apparatus and methodology changes that are detailed here.

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Immunology and Infection

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Jin Gao 1, Laura Couzens 1, Maryna C. Eichelberger 1
1Division of Viral Products, CBER, Food and Drug Administration

We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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Medicine

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
Jessica Felton 1, Kunrong Cheng 2, Anan Said 2, Aaron C. Shang 2, Su Xu 3, Diana Vivian 4, Melissa Metry 5, James E. Polli 5, Jean-Pierre Raufman 2,6
1Department of Surgery, University of Maryland School of Medicine, 2Department of Medicine, University of Maryland School of Medicine, 3Department of Radiology, University of Maryland School of Medicine, 4Food and Drug Administration, 5Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 6VA Maryland Health Care System

Tools to diagnose bile acid malabsorption and measure bile acid transport in vivo are limited. An innovative approach in live animals is described that utilizes combined proton (1H) plus fluorine (19F) magnetic resonance imaging; this novel methodology has translational potential to screen for bile acid malabsorption in clinical practice.

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