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The Walter and Eliza Hall Institute of Medical Research

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

Victoria Ryg-Cornejo¹, Lisa J. Ioannidis¹, Diana S. Hansen¹

¹The Walter and Eliza Hall Institute of Medical Research

A method for isolation of adherent inflammatory leukocytes from brain blood vessels of Plasmodium berghei ANKA-infected mice is described. The method allows quantification as well as phenotypic characterization of isolated leukocytes after staining with fluorescent antibodies and subsequent analysis by flow cytometry.

Chemistry

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Pooja Sharma^1,2, Mariam Kaywan-Lutfi¹, Logesvaran Krshnan^1,2, Eamon F. X. Byrne^1,2, Melissa Joy Call^1,2, Matthew Edwin Call^1,2

¹Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, ²The University of Melbourne

Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.